THE SATURATION CHARACTERISTICS OF GLUCOSE TRANSPORT IN BOVINE RETINAL PIGMENT EPITHELIUM

• 来源期刊: 眼科学报
• 学科: 医学
• 年，卷（期）: 1998,（3）
• 页码范围: 126-129
• 页数: 4页
• 分类号: R770.2
• 语种: 中文

摘要

<正> Purpose: To study the saturation characteristics of the glucose transport across the bovine retinal pigment epithelium(RPE).Methods :The bovine RPE preparations were munted with a modified Ussing chamber. The L-[3H]-glucose and 3-0-methyl-D-[14C]-glucose fluxes across the RPE from the choroid to retina were studied at different glucose concentrations.Results: The glucose transport was found to be stereospecific, with 3-0-methyl-D-glucose (MDG) being transported about three times faster than L-glucose. The glucose transport showed typical saturation characteristics in Michaelis-Menten fashion. The Vmax and the Km of corrected MDG were 2452 nmol cm-2h-1 and 30.8 mM respectively. It was shown that the glucose transport system was saturated at 61.6 mM.Conclusions : The saturation characteristics of the corrected MDG flux suggested that the capacity of glucose transport through the bovine RPE is immense. Eye Science 1998; 14: 726 -729.