PROPERTIES AND SYNTHESIS OF NEW SUPPORTS FOR IMMOBILIZATION OF ENZYMES BY COPOLYMERIZATION OF VINYLENE CARBONATE AND METHACRYLIC ACID

• 来源期刊: 高分子科学（英文版）
• 年，卷（期）: 2000,（4）
• 页码范围: 343-349
• 页数: 7页
• 语种: 英文

摘要

Methacrylic acid first was neutralized with an aqueous solution of NaOH to pH = 6.0～7.0, vinylene carbonate (VCA) was added to the solution, then monomers were copolymerized in paraffin oil by means of reverse-phase suspension polymerization and hydrophilic copolymeric supports were prepared. The properties of the supports were determined using trypsin and results show that the amount of enzymes coupled to the supports and the specific activity of immobilized trypsin are related to the content of VCA structure units, reaction time and concentration of enzyme solution, etc.