EXPRESSION OF THE GENE ENCODING THE TETRAPLOID OF CARBOXYL-TERMINAL PEPTIDE OF β-HCG CONTAINING THIRTYSEVEN AMINO ACID RESIDUES IN E.COLI

• 来源期刊: 生殖与避孕(英文版)
• 学科: 医学
• 关键词: 清平
• 年，卷（期）: 2000,（3）
• 页码范围: 113-119
• 页数: 7页
• 分类号: R394.8
• 语种: 中文

摘要

This study was carried out to investigate the possible enhancement of im- munogenicity of the carboxyl-terminal peptide of β-hCG which is made up of 37 amino acid residues (109-145) and contains the specific epitope (antigenic determinant) of hCG. Materials &. Methods hCGβ-CTP37 tetraploid cDNA was constructed by linking four hCGβ-CTP37 cDNAs together. The product was then subcloned into the E. coli expres- sion vector pQE60 to construct the expression vector pQE60/ (hCGβ-CTP37)4. Recom- binant (hCGβ-CTP37)4 was expressed in E. colt-X-blue. Results Western blot analysis showed that the tetraploid of hCGβ-CTP37 had an ap- parent molecular weight of 20 kD and had relatively stronger anti-hCG antibody-bind- ing activity compared with the diploid from. Conclusion The tetraploid of hCGβ-CTP37 may be a more potent immunogen for raising anti-hCG vaccines for fertility regulation or suppression of tumor.