STUDY ON THE REGULATION OF BCL-2 GENE ON RAT SPERMATOGENIC CELLS APOPTOSIS IN TRANSCRIPTION LEVEL

• 来源期刊: 生殖与避孕(英文版)
• 学科: 生物学
• 关键词: 震波
• 年，卷（期）: 2000,（4）
• 页码范围: 187-193
• 页数: 7页
• 分类号: Q954.4
• 语种: 中文

摘要

Objective To detect the change of Bcl 2 gene expression in the apopototic process of spermatogenic cells in rat with vasoligation and vasostomy, and to find out the relationship between the transcription of Bcl 2 and the apoptosis of spermatognic cells Materials & Methods Sixty adult male Sprague Dawley rats in 3 groups were operated with vasoligation and vasostomy. Then hybridization in situ with hypersensitive Bcl 2 RNA probe was used to detect the change of Bcl 2 mRNA. Results The transcription of Bcl 2 gene in spermatogenic cells was obviously inhibited in the vasoligation group compared with that in the control group (P<0.05), and the transcription in the vasostomy group showed no difference from that of the control group. Conclusion Bcl 2 gene has an anti apoptotic effect in rats with vasostomy, and there was a transcriptional regulation of Bcl 2 gene in rat spermatogenic cell during the period of pre vasoligation to post vasoligation and to post vasosotomy.