脊髓切片中运动神经元的膜片箝技术研究：一种分析药物作用的高效工具

• 来源期刊: 中国药理学报(英文版)
• 关键词: 脊髓 运动神经元 乙醇 醇类 全身麻醉 突触后机制
• 年，卷（期）: 2000,（6）
• 所属期刊栏目: 研究原著
• 页码范围: 507-515
• 页数: 9页
• 语种: 英文

摘要

AIM: To develop a tool for detailed analysis of spinally acting anesthetic and analgesic agents. METHODS:Studies were done on visually identified motor neurons in 400 μm thick spinal cord slices from 14- 23 d old rats using patch clamp techniques. Ethanol was used as a prototype general anesthetic agent. RESULTS: Cell bodies in the ventrolateral horn identified as motor neurons by retrograde fluorescent labeling had a mean dimension of 32 ±5μm (x±s, n = 25). Mean resting potential was - 62.8 ± 2.4 mV; input resistance was 44±24MΩ (n=19). Threshold was -44±7 mV,and action potential amplitude 101 ± 9 mV from baseline.Ethanol concentrations at and below 50-200 mmol/L decreased motor neuron excitability to the injected current; there was no effect on resting potential, but a variable reversible increase in input resistance. Ethanol reversibly depressed the excitatory postsynaptic potential,with a dose-response relationship similar to that previously observed for the population excitatory postsynaptic potential in intact spinal cord in vitro. Ethanol also reversibly depressed currents evoked by glutamate, reducing total charge transfer to 40％±26％ of control (x±s; n= 4). CONCLUSION: Reduction of connectivity in this relatively thick slice preparation does not significantly modify drag actions. The actions of ethanol on excitatory synaptic transmission observed in intact spinal cord are