N-乙酰半胱氨酸和L-NAME对磷化铝诱导的大鼠心血管毒性的作用

• 作者: Archana AZAD Shivani MITTRA Shyam Bala LALL
• 来源期刊: 中国药理学报(英文版)
• 学科: 医学
• 关键词: 铝化合物 乙酰半胱氨酸 NG-硝基精氨酸 甲酯 丙二醛 过氧化氢酶 谷胱甘肽过氧化酶
• 年，卷（期）: 2001,（4）
• 所属期刊栏目: 研究原著
• 页码范围: 298-304
• 页数: 7页
• 分类号: R96
• 语种: 英文

摘要

AIM: To investigate the protective effects of N-acetyl- cysteine (NAC) and Nω-Nitro-L-arginine methyl ester (L-NAME) on aluminium phosphide (AlP) poisoning induced hemodynamic changes, myocardial oxygen free radical injury and on survival time in rats. METH- ODS: AlP (12.5 mg/kg) was administered intragastri cally under urethane anaesthesia. The effect of pre- and post-treatment with NAC and L-NAME alone and in combination was studied on haemodynamic parameters [blood pressure (BP), heart rate (HR), and electrocar- diogram (ECG) ] and biochemical parameters ( malonyl- dialdehyde, catalase, and glutathione peroxidase). RE SULTS: AlP caused significant hypotension, tachycar dia, ECG abnormalities, and finally marked bradycardia. The mean survival time was (90 ± 10) min. There was significant increase in myocardial malonyldialdehyde (MDA), and decrease in catalase and glutathione peroxi dase (GSH Px) levels. NAC infusion (6.25 mg·kg-1· min-1, iv for 30 min) caused insignificant hemodynamic and biochemical changes. Pre- and post-treatment of NAC with AlP significantly increased the survival time, stabilized BP, HR, and ECG, decreased MDA and in creased GSH Px levels compared to AlP group. L- NAME infusion ( 1 mg· kg- 1· min- 1, iv for 60 min) as such caused significant rise in BP but precipitated ECG abnormalities. Pre- and post-treatment of L-NAME with AlP neither improved the survival time nor the biochemi cal parameters despite significant rise in BP. Co-admin- istration of both the drugs with AlP worsened the hemo dynamic and biochemical parameters with reduction in the survival time as compared to AlP. CONCLUSION: NAC increased the survival time by reducing myocardial oxidative injury whereas L-NAME showed no such pro tective effects in rats exposed to AlP.