THE BINDING ABILITY ANALYSIS OF THE NORMAL VLDL RECEPTOR AND ITS MUTANT

• 来源期刊: 同济医科大学学报(外文版)
• 学科: 医学
• 关键词: 屈伸
• 年，卷（期）: 2001,（3）
• 页码范围: 177-180,194
• 页数: 5页
• 分类号: R329.2
• 语种: 中文

摘要

The ligand-binding domain of VLDL receptor contains eight imperfectly similar repeats. To discuss the contribution of each repeat to ligand binding, the RT-PCR technique was used to clone the VLDLR-cDNA from the heart muscle of Chinese people. Two recombinants were further constructed, which contained the full-length cDNA of VLDLR and the mutant lacking repeats 1-5. CHO cell line was transfected with two recombinants. The expression of VLDLR gene could be detected by RT-PCR from the CHO cells transfected with pCD-VR. The results of binding experiments showed that the ability of the CHO cells transfected with the full-length cDNA of VLDL-R binding DiI-labeled β-VLDL was higher than that of the CHO cells transfected with the mutant. Our findings indicated that human VLDL-R gene could be expressed effectively on CHO cells, and the receptor was almost inactivated when repeats1-5 were deleted.