CLONING AND IDENTIFICATION OF A NOVEL VARIANT OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR

• 来源期刊: 同济医科大学学报(外文版)
• 学科: 医学
• 关键词: 屈伸
• 年，卷（期）: 2001,（3）
• 页码范围: 191-194
• 页数: 4页
• 分类号: R329-33
• 语种: 中文

摘要

A novel variant of human vascular endothelial growth factor (h'VEGF165) cDNA was amplified by nested PCR method from the HL60 1 cells and was cloned into a eukaryotic expressing vector pcDNA 3 to construct a recombinant plasmid pCD-h'VEGF165. The amplified h'VEGF165 cDNA fragment was identified by enzyme digestion and DNA sequencing methods. Also, wild-type hVEGF165 cDNA was obtained, identified and cloned into a eukaryotic expressing vector pcDNA3 by using the same methods. The results of DNA sequencing showed that h'VEGF165 cDNA cloned from HL60 1 was 600 bp in size with 8 % of the base sequence in h'VEGF165 cDNA being changed as compared with the base sequence in the wild-type hVEGF165 cDNA. The results of sequencing of hVEGF165 which was cloned from HL60 by us were consistent with the reports completely.