EXPRESSION OF P53 DURING LENS EPITHELIAL CELL APOPTOSIS INDUCED BY ULTRAVIOLET

• 来源期刊: 同济医科大学学报(外文版)
• 学科: 医学
• 年，卷（期）: 2001,（3）
• 页码范围: 263-264
• 页数: 2页
• 分类号: R329
• 语种: 中文

摘要

The apoptosis of lens epithelial cells (LECs) induced by ultraviolet and the expression of P53 were investigated. Wistar rats received 100 mW/m 2 ultraviolet irradiation (UVR) (λ=280 nm-315 nm) for 15 min. One, 6, 24 h after irradiation the lens capsules were dissected. The percentages of apoptotic cells were evaluated by the TdT-dUTP terminal nick-end labeling (TUNEL) technique and the expression of P53 was detected by using immunohistochemical assay. The results showed that the percentages of TUNEL-positive nuclei at 24 h after irradiation was significantly higher than in the control group and those 1 h, 6 h after irradiation. The percentages of P53-positive cells at 6 h, 24 h after irradiation were significantly higher than in the control group and those 1 h after irradiation. It was concluded that UVR could induce the apoptosis of lens epithelial cell. The expression of P53 might be responsible for the apoptosis of lens epithelial cells.