氦氖激光诱导培养瘢痕成纤维细胞凋亡的实验研究

• 来源期刊: 现代康复
• 学科: 医学
• 关键词: aser He-Ne hypertrophicscar fibroblast apoptosis
• 年，卷（期）: 2001,（20）
• 所属期刊栏目: 中国康复医学快报英文专栏
• 页码范围: 148-149
• 页数: 2页
• 分类号: R6
• 语种: 中文
• DOI: 10.3321/j.issn:1673-8225.2001.20.147

摘要

Objective To explore He- Ne laser inducing apoptosis of cultured fibroblasts in hypertrophic scars(HS).Methods Cultured fibroblasts in HS were irradiated with He- Ne laser. Apoptosis was examined by TUNEL technique and cell necrosis was counted by trypan blue staining. Results There was no cell apoptosis after repeated irradiation with 10mW/cm2 and 50mW/cm2 for 10 and 30 minutes. The percent of apoptosis was 7.20% and 12.73% after repeated irradiation with 100mW/cm2 for 10 and 30 minutes. The percent of apoptosis was 14.74% and 16.27% after laser irradiation with 150mW/cm2 for 10 and 30 minutes.No necrotic cell was presented after repeated irradiation for 10 and 30 minutes at various power densities. Conclusion Repeated He- Ne irradiation of HS- derived fibroblast cultures at power densities of 100mW/cm2 and 150 mW/cm2 can induce cell apoptosis. Power density is more important than energy density so far as laser- inducing apoptosis of scar fibroblasts concerned.