Transient Expression of Human Papillomavirus Type 16 (HPV 16) mRNA in Normal Human Keratinocvtes Transfected by pSV2-neo/16

• 来源期刊: 中华性传播感染杂志（英文版）
• 学科: 医学
• 关键词: Papillomavirus Human Plasmid Keratinocytes Transfection
• 年，卷（期）: 2002,（2）
• 页码范围: 12-15
• 页数: 4页
• 分类号: R511
• 语种: 英文

摘要

Objectives: To investigate the expression of HPV16 mRNA innormal human keratinocytes transfected with pSV2-neo/16.Methods: First human keratinocytes were cultured in theserum-free medium M154.Second, the plasmid pSV2-neo/16was transfected into the human keratinocytes using atransfecting reagent. Third, RT-PCR and Southern Blottingwere used to detect the expression of HPV16 mRNA and DNAin the transfected keratinocytes, respectively.Results: The expression of HPV 16 mRNA was successfullyamplified and an 110bp was detected by RT-PCR. A 7.9kbfragment was confirmed in the transfected keratinocytes bySouthern Blot analysis.Conclusion: HPV 16 mRNA and DNA were successfullydetected in the human keratinocytes.