Antigen Gene Cloning and Expression of HIV-1 Toward an AIDS Vaccine DesignⅠ. Amplification and Sequencing of HIV-l Antigen Genes

• 来源期刊: 中华性传播感染杂志（英文版）
• 学科: 医学
• 关键词: HIV-1 Antigen gene Sequencing Subtype
• 年，卷（期）: 2002,（1）
• 页码范围: 1-6
• 页数: 6页
• 分类号: R511
• 语种: 英文

摘要

Objective:To amplify antigen genes from patients withhuman immunodeficiency virus type 1 (HIV-1) in GuangdongProvince for candidate AIDS vaccine design.Methods: Viral nucleic acid was isolated from 10 HIV-1infected individuals' peripheral blood collected during1995-2000 in Guangdong Province. The viral gag p24 geneand env gp120 gene were amplified by nested-PCR andsequenced. The homologies among HIV-1 isolates werecompared with HIV-BLAST.Results: Among 10 HIV-1 isolates, nine are homologous toviruses of subtype B, and one is homologous to viruses ofsubtype E.Conclusion: Subtype B viruses of HIV-1 are predominantlypresent in Guangdong Province.