STUDIES ON BLOOD GLUTATHIONE PEROXIDASE PROTEIN LEVEL OF CHILDREN IN KESHAN DISEASE AND KASHIN-BECK DISEASE AREAS

• 作者: Guanglu(徐光禄) Lianbang(翟连榜) Lixin(王立新) Tan Wang Wuhong(谭武红) Xiaohong(种晓红) Xu Yang Zhai Zhantian(杨占田) Zhong
• 来源期刊: 西安交通大学学报：英文版
• 学科: 生物学
• 关键词: Kashan DISEASE Kashin-Beck DISEASE CHILDREN GLUTATHIONE PEROXIDASE PROTEIN
• 年，卷（期）: 2002,（2）
• 页码范围: 133-135
• 页数: 3页
• 分类号: Q503

摘要

Objective To oberve the change in blood glutathione peroxidase (GSH-Px) protein levels of residents in the low-selenium (Se) area by contrasting the blood GSH-Px protein level of the children in the Keshan disease area with those in the Kashin-Beck disease and non-endemic areas. Methods GSH-Px protein levels were measured by enzyme-linked immunosorbent assays (ELISA). The Se content and GSH-Px activity were assayed by the 2,3-diaminonaphthalene spectrofluorimetric method and glutathione reductase-coupled method respectively. Results ①The blood Se content and GSH-Px protein level of children in Keshan disease area (Moding) were significantly lower than those in Xi'an non-endemic area, however, there was no significant difference when compared with the low-Se non-endemic area; ②The blood Se content, GSH-Px activity and GSH-Px protein level of children in the Kashin-Beck disease area (Yulin) were significantly lower than those of children in two non-endemic areas and in the Keshan disease area; ③The blood Se content and GSH-Px activity were positively correlated to the GSH-Px protein level respectively. Conclusion These results indicate that the blood GSH-Px protein level is decreased in the low-Se residents. The Se status not only affects the GSH-Px activity but also regulate the GSH-Px protein level.