Molecular Detection,Culture and Isolation of Mycoplasma Pneumoniae From Reproductive Tract of STD Patients

• 来源期刊: 中华性传播感染杂志（英文版）
• 学科: 医学
• 关键词: Mycoplasma Pneumoniae PCR DNA sequence Determination
• 年，卷（期）: 2002,（3）
• 页码范围: 41-45
• 页数: 5页
• 分类号: R75
• 语种: 英文

摘要

Objective: To confirm whether Mycoplasma pneumoniae(MP) are present in reproductive tract of STD patients in China.Methods: Application of nested PCR (nPCR) and DNA sequencing to test samples of urethral/vaginal swabs withMP culture confirmation of several nPCR positive patients.Results: 74 of 786 STD patients were positive for MP bynPCR, with a rate of 9.4%. Of the 484 male patients, 10.5%were positive, and among the 302 female patients, 7.6%were positive. There was no significant difference betweenthem (P＞0.05). Of 12 cases of MP positive samples by nPCR,4 cases were first generation culture-positive, and one ofthem passed to the next generation successfully. DNAsequencing was performed on the nPCR product of oneswab sample and one MP culture isolation. The determinedsequence was identical to the typical MP strain.Conclusion: In China, MP are present in reproductivetract of both male and female STD patients.