Cloning of Omp1 Gene from Chlamydia trachomatis F Genotype

• 来源期刊: 中华性传播感染杂志（英文版）
• 学科: 生物学
• 关键词: Chlamydia trachomatis F genotype main outermembrane protein omp1 gene clone DNA vaccine
• 年，卷（期）: 2002,（2）
• 页码范围: 25-28
• 页数: 4页
• 分类号: Q7
• 语种: 英文

摘要

Objectives: To directionally clone the omp1 gene fromChlamydia trachomatis (Ct) F genotype onto a plasmid vectorfor constructing a rudimentary DNA vaccine.Methods: The complete omp1 gene from genomic DNA of CtF genotype wild species was amplified with primers designedby computer. The recombinant gene was obtained byrestriction enzyme cutting, linking the gene with the plasmidvector in vitro, transforming the recombinant gene intobacteria, and extracting the DNA from the bacteria.Results: DNA extracted from the bacteria was composed ofthe omp1 gene and plasmid, which is identified by threemethods of singular restrictive enzyme cutting, doublerestrictive enzyme cutting and PCR.Conclusion: Cloning of the omp1 gene from the Ct Fgenotype means that a rudimentary DNA vaccine wassuccessfully constructed.