Detection of Tumor Necrosis Factor Gene by Piezoelectric Nucleic Acid Sensor

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2002,（10）
• 页码范围: 1044-1048
• 页数: 5页
• 语种: 英文

摘要

A piezoelectric nucleic acid sensor was constructed for detection of tumor necrosis factor gene. Two methods were employed for immobilization of nucleic acid probe on gold electrode of piezoelectric crystal. The results show that polyethyleneimine adhesion and glutaraldehyde cross-linking method has higher sensitivity, stability and selectivity than protein A method. The solid-phase nucleic acid hyb indization of oligo nucleotides and tumor necrosis factor target gene sequence were monitored using this sensor. Tumor necrosis factor gene sequence (580 bp) was detected by this nucleic acid sensor for the first time.