Monitoring the Hydrolysis of p-Nitrophenyl Acetate Catalyzed by Seryl-histidine with Electrospray Ionization Mass Spectrometry

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2002,（10）
• 页码范围: 1097-1101
• 页数: 5页
• 语种: 英文

摘要

The hydrolysis of p-nitrophenyl acetate (p-NPA) catalyzed by seryl- histidine or histidine has been monitored by electrospray ionization mass spectrometry in the presence of the internal calibration, 8-anilino-1-naphthalenesulfonic acid ammonium salt (ANS). The half-life of p-NPA in the presence of 10 mmol.L-1 seryl- histidine or histidine at25 ℃ was 370 min and70 min respectively. With the occurrence of acetyl seryl- histidine and acetyl histidine in the reaction, and the fact that p-NPA was stable in the presence of 10 mmol. L- 1 serine, an imidazolysis mechanism has been proposed, which is in accordance with the reported work.