He-Ne激光重复照射抑制培养增生性瘢痕成纤维细胞胶原合成的实验研究

• 作者: 杨宏珍 杨西川
• 来源期刊: 中国临床康复
• 学科: 医学
• 关键词: He Ne laser hypertrophic scar fibroblast collagen synthesis type I procollagen mRNA
• 年，卷（期）: 2002,（2）
• 所属期刊栏目: English Column(英文专栏)
• 页码范围: 296-297
• 页数: 2页
• 分类号: R619
• 语种: 中文

摘要

Objective To explore the inhibitory effects of He Ne laser repeated irradiation on the collagen synthesis of cultured scar fibroblasts. Method Cultured fibroblasts derived from hypertrophic scars(HS) were irradiated with He Ne laser for 30 minutes at various power densities(10,50,100 and 150 mW/cm2),once a day for 3 consecutive days.In 24 hours after repeated irradiation collagen production and type I procollagen mRNA level of fibroblasts were measured with the incorporation of 3H proline and blot hybridization techniques espectively.Results Collagen synthesis and type I procollagen mRNA level remained unchanged when the laser was irradiated at the power density of 10 mW/cm2 or 50 mW/cm2.Compared with control,collagen synthesis and type I procollagne mRNA level were significantly decreases at the power density of 100 mW/cm2 or 150mW/cm2(P< 0.05).Type I procollagen mRNA level at the power densityof 150 mW/cm2 was lower than that at the 100 mW/cm2 (P< 0.05).Conclusion Repeated He Ne laser irradiation at the power density of 100 mW/cm2 or 150 mW/cm2 can suppress collagen synthesis of cultured fibroblasts in HS.The cause of suppression may be associated with down regulation of type I procollagen mRNA expression.