ESTIMATE OF OXYGEN CONSUMPTION AND INTRACELLULAR ZINC CONCENTRATION OF HUMAN SPERMATOZOA IN RELATION TO MOTILITY

• 来源期刊: 亚洲男性学杂志(英文版)
• 学科: 医学
• 年，卷（期）: 2003,（1）
• 页码范围: 3-8
• 页数: 6页
• 分类号: R33
• 语种: 中文

摘要

Aim: To investigate the human sperm oxygen/energy consumption and zinc content in relation to motility. Methods: In washed spermatozoa from 67 ejaculates, the oxygen consumption was determined. Following calculation of the total oxygen consumed by the Ideal Gas Law, the energy consumption of spermatozoa was calculated. In addition, the zinc content of the sperm was determined using an atomic absorption spectrometer. The resulting data were correlated to the vitality and motility. Results: The oxygen consumption averaged 0.24μmol/106 sperm*24 h, 0.28μmol/106 live sperm*24 h and 0.85μmol/106 live & motile sperm*24 h. Further calculations revealed that sperm motility was the most energy consuming process (164.31 mJ/106 motile spermatozoa*24 h), while the oxygen consumption of the total spermatozoa was 46.06 mJ/106 spermatozoa*24 h. The correlation of the oxygen/ energy consumption and zinc content with motility showed significant negative correlations (r= -0.759; P<0.0001 and r=-0.441; P<0.0001, res