ANDROGEN-INDEPENDENT GROWTH IN LNCAP CELL LINES AND STEROID URIDINE DIPHOSPHATE-GLUCURONOSYLTRANSFERASE EXPRESSION

• 来源期刊: 亚洲男性学杂志(英文版)
• 学科: 生物学
• 年，卷（期）: 2003,（1）
• 页码范围: 9-13
• 页数: 5页
• 分类号: Q57
• 语种: 中文

摘要

Aim: To investigate the mechanism of androgen-independent growth of prostate cancer after androgen ablation in LNCaP cells and the effect of glucuronidation activity. Methods: To establish androgen-independent growth in prostate cancer LNCaP-SF, continuous passage was performed in androgen-stripped medium and the cells were evaluated for glucuronidation activity. The expression vector of antisense uridine diphosphate glucuronosyltransferase (UGT) 2B15 cDNA was also constructed and evaluated. Results: LNCaP-SF lead to a higher expression in UGT2B15 and their glucuronidation activity is 2.5 times higher than that of LNCaP cells. Significantly fewer LNCaP and LNCaP-SF than control were transfected with the antisense UGT2B15 cDNA, suggesting that UGT2B15 plays an important part in the glucuronidation activity of androgens in both cells. Conclusion: The alteration of UGT2B15 expression in LNCaP-SF cells is proposed as a biological characteristic involved in the growth of hormonerefractory prostate cancer.