TARGETED EXPRESSION OF HUMAN FSH RECEPTOR ASP567GLY MUTANT MRNA IN TESTIS OF TRANSGENIC MICE: ROLE OF HUMANFSH RECEPTOR PROMOTER

• 来源期刊: 亚洲男科学杂志(英文版)
• 学科: 医学
• 年，卷（期）: 2003,（4）
• 页码范围: 267-275
• 页数: 9页
• 分类号: R346
• 语种: 中文

摘要

Aim: To specifically express the Asp567Gly human follicle-stimulating hormone receptor (FSHR) under the control of its promoter to evaluate the phenotypic consequences in the presence of normal pituitary function. Methods: We produced transgenic mice overexpressing the Asp567Gly human FSHR under the control of a 1.5kb 5' flanking region fragment of its promoter. Results: Mice were phenotypically normal and fertile. In males, mRNA could be detected in the testis and the brain, indicating that the 1.5kb promoter fragment drives expression not only in the gonads. The testis weight/body weight ratio and the testosterone levels in transgenic and non-transgenic litter mates were similar. By in situ hybridisation we found that the transgenic FSHR was highly expressed in Sertoli cells, spermatocytes and round spermatids. However, a radioligand receptor assay failed to show a significant difference in total FSHR binding sites in testis homogenates of transgenic and wild type animals, suggesting that the transgeni