多形核白细胞导致血管内皮细胞通透性增高的机理

• 作者: Sahra Y. 崔莉莉 张涛 徐波 李正军 马维娅
• 来源期刊: 中华现代临床医药杂志
• 学科: 医学
• 关键词: Neutrophils Endothelial Permeability β- Catenin VE- Cadherin Tyrosine Phosphorylation
• 年，卷（期）: 2003,（6）
• 所属期刊栏目: 论著
• 页码范围: 1-3
• 页数: 3页
• 分类号: R74
• 语种: 中文

摘要

Objective We investigated the effect of neutrophil(PMNs) activation on vascular endothelial cells (CVECs)integrity. Methods The methods of Western Blot analysis and the immunocytochemistry were used to survey the expressionand morphologic changes for tyrosine phosphorylation of adhesive proteins β - Catenin and VE - Cadherin in CVECs. ResultsThe results showed that activated PMNs induced a significant increase in tyrosine phosphorylation of β- Catenin and VE -Cadherin. The immunofluorescent staining showed a widespread gap formation and a loss of β- Catenin and VE- Cadherinstaining at the CVECs periphery after the cells were treated with activated PMNs. Conclusion These data suggest thatneutrophils may regulate endothelial barrier function through a process conferring conformational changes to tyrosinephosphorylation of β- Catenin and VE- Cadherin.