短暂脑缺血诱导成年大鼠纹状体内CRMP-4的表达

• 作者: 刘鹏翀 孙凤艳 张玲妹 杨增进 邱梅红
• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: brain ischemia corpus striatum CRMP-4 protein intermediate filament proteins bromodeoxyuridine neurons regeneration
• 年，卷（期）: 2003,（12）
• 所属期刊栏目: 研究原著
• 页码范围: 1205-1211
• 页数: 7页
• 分类号: R74
• 语种: 英文

摘要

AIM: To study the expression of collapsing response mediated protein-4 (CRMP-4) and nestin in the ischemic adult rat brain following transient brain ischemia. METHODS: Brain ischemia was induced by transient left middle cerebral artery occlusion (MCAO) for 60 min in adult rats. The expression of CRMP-4, nestin and bromodeoxyuridine (BrdU) was analyzed by immunohistochemical method. The co-localization of CRMP-4 and nestin or BrdU was analyzed by double staining combined with confocal laser scanning microscopy. RESULTS: CRMP-4, a marker of immature neuron, could be expressed in the ipsilateral striatum and cerebral cortex at 1st and 2nd week after the ischemia-reperfusion; nestin, a marker of neural stem cell, occurred in above regions from several hours to 2 weeks. CRMP-4 costained with nestin and with BrdU incorporation. CONCLUSION: Neural stem cells may present in the striatum and cerebral cortex of adult rat and can be triggered to differentiate into newborn neuron there by ischemic brain trauma.