摘要:
目的:探讨吲哚美辛(Indomethacin,IN)对体外培养的人胚胎视网膜色素上皮(RPE)细胞的抑制作用及DNA合成的影响.方法:分别用MTT法测定加入100 μ mol/L1,200 μ mol/L1,400 μ mol/L1,600μ mol/L1,800 μ mol/L1,1000μ mol/L1IN12h后RPE细胞数量的改变和核酸蛋白分析仪测定加入50 μ mol/L1,100μ mol/L1,200 μ mol/L1,400 μ mol/L1,600 μ mol/L1IN作用24h后RPE细胞DNA浓度的变化.结果:100 μ mol/L1,200μmol/L1,400 μ mol/L1,600μ mol/L1,800μ mol/L1,1000 μ mol/L1IN作用12h后,其细胞A值分别为(0.2367±0.0564),(0.1687±0.0695),(0.0819±0.0346),(0.0656±0.0176),(0.0554±0.0286),(0.0508±0.0277)与对照组(0.2674±0.0430)相比,差异有显著性(t值分别为1.442,4.621,8.682,9.447,9.925,10.140,P值分别为0.158,0.000,0.000,0.000,0.000,0.000);50 μ mol/L1,100 μ mol/L1,200μ mol/L1,400 μ mol/L1,600 μ mol/L1IN作用48h后其细胞DNA浓度分别为(101.1712±15.5124),(88.6400±13.5845),(72.3651±7.7969),(5.9089±10.7229),(51.2236±8.7757)μ g/ml1,对照组(213.7351±83.1572)μ g/ml1相比,差异有显著性(t值分别为5.481,6.091,6.883,7.490,7.912,P值分别为0.000,0.000,0.000,0.000,0.000).结论:IN可抑制体外培养的RPE细胞增殖及促进细胞DNA合成.