Synthesis of Monodisperse Poly(glycidylmethacrylate-co-ethylene dimethacrylate) Beads and Their Application in Separation of Biopolymers

• 来源期刊: 中国化学（英文版）
• 学科: 化学
• 年，卷（期）: 2004,（3）
• 页码范围: 283-289
• 页数: 7页
• 分类号: O6
• 语种: 英文

摘要

The monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads with macroporous in the range of 8.0-12.0 μm were prepared by a single-step swelling and polymerization method. The seed particles prepared by dispersion polymerization exhibited good absorption of the monomer phase. The pore size distribution of the beads was evaluated by gel permeation chromatography and mercury intrusion method. By using this media, a weak cation exchange (WCX) stationary phase for HPLC was synthesized by a new chemical modification method. The prepared resin has advantages of biopolymer separation, high column efficiency, low column backpressure, high protein mass recovery and good resolution for proteins. The measured bioactivity recovery for lysozyme was (96±5)%. The dynamic protein loading capacity of the synthesized WCX packings was 21.3 mg/g. Five proteins were completely separated in 8.0 min using the synthesized WCX stationary phase. The experimental results show that the obtained WCX resin has very weak hydrophobicity. The WCX resin was also used for the rapid separation and purification of lysozyme from egg white in 8 min with only one step . The purity and specific bioactivity of the purified lysozyme was found more than 92.0% and 70184 U/mg, respectively.