Novel Rearrangement of Small Peptides in Electrospray Ionization Tandem Mass Spectrometry
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摘要:
Electrospray ionization mass spectrometry (ESI-MS) is a powerful method for sequencing peptides.A novel fragmentation pattern with the loss of a neutral fragment of 45 Da was observed with the dipeptides,tripeptides,tetrapeptides and pentapeptides containing phenylalanine or histidine residues.A novel rearrangement reaction with the extrusion of a formamide piece was studied and the rearrangement mechanism was proposed and confirmed by deuterium labeling experiments with ESI-MSn and high-resolution mass spectrometry.These findings are potentially helpful in identifying the specific sequence pattern in the peptide sequencing.