Detection of Hepatitis B Virus DNA by Duplex Scorpion Primer-based PCR Assay

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2004,（9）
• 页码范围: 903-907
• 页数: 5页
• 语种: 英文

摘要

The application of a new fiuorogenic probe-based PCR assay (PCR duplex scorpion primer assay) to the detection of Hepatitis B virus (HBV) DNA in human sera was described. Duplex scorpion primer is a modified variant of duplex Amplifluor, and the incorporation of a PCR stopper between probe and primer sequences improve the detection specificity and sensitivity. Combined with PCR amplification, this probe can give unambiguous positive results for the reactions initiated with more than 20 HBV molecules. In addition, the particular unimolecular probing mechanism of this probe makes the use of short target-specific probe sequence possible, which will render this probe applicable in some specific systems.