托吡酯对Lewis肺癌转移的抑制作用与水通道蛋白<em>1</em>的关系

• 作者: Yang XIANG 于和鸣 李学军 李涛 马兵
• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: topiramate aquaporins tumor metastasis carbonic anhydrase
• 年，卷（期）: 2004,（1）
• 所属期刊栏目: 研究原著
• 页码范围: 54-60
• 页数: 7页
• 分类号: R73
• 语种: 英文

摘要

AIM: To study the effect of topiramate on tumor metastasis and its relation with aquaporin 1 (AQP1) water channel. METHODS: Lewis lung carcinoma metastatic model was used to determine the effect of topiramate on tumor growth and metastasis. Colorimetric estimation was used to investigate the action of topiramate on carbonic anhydrase (CA) activity. Western blotting and immunohistochemical analysis were used to study the influence of topiramate on AQP1 water channel expression in lungs or tumor tissues of mice bearing Lewis lung carcinoma.cantly (P＜0.05). Its inhibitory rate of metastasis was 81.25 %. Topiramate inhibited CA activity in lungs of mice in a dose-dependent manner. Topiramate apparently decreased AQP1 protein expression and immunostaining in lungs or in tumor microvessel endothelial cells of mice. CONCLUSION: Suppression of AQP1 water channel expression may be an important pathway for the inhibitory effect of topiramate on tumor metastasis.