Separating Kanamycin A And B From The Fermentation Broth

• 来源期刊: 中国新医药
• 学科: 医学
• 关键词: Kanamycin equilibrium constants resins
• 年，卷（期）: 2004,（4）
• 所属期刊栏目: 医药研究(论著与综述)
• 页码范围: 37-38
• 页数: 2页
• 分类号: R9
• 语种: 中文

摘要

A method to separate Kanamycin A and B from their fermentation broth is proposed in this paper. In order to seek Kanamycin B-sensitive resin, the equilibrium constants of Kanamycin B were estimated by potentiometric titration, and then the resins for test were evaluted by static and dynamicabsorption. The results indicated that the D4 with larger mesh has higher adsorption rate of Kanamycin B and lower adsorption amount of impurity. Using D4 as first column and 732# as late binding columns, the purity of Kanamycin A is higher than 99.0%.