Co-localization of histamine and norepinephrine in sympathetic ganglia and exocytosis of endogenous histamine from cardiac sympathetic nerve endings of macaca mulatto monkey

• 作者: JiaMENG Jing-ruMENG JingHU Liang-weiCHEN Ming-kaiLI Xiao-xingLUO XueMA Yu-meiWU ZhengHOU ZhongCHEN
• 来源期刊: 中国药理通讯
• 学科: 医学
• 关键词: Co-定位 组胺 去甲肾上腺素 交感神经 神经中枢 细胞外分泌 内生性组胺 强心剂 混血猴 神经系统用药
• 年，卷（期）: 2005,（2）
• 页码范围: 18
• 页数: 1页
• 分类号: R971

摘要

AIM To provide the evidence about localization, biosynthesis, metabolism and release of histamine from the cardiac sympathetic nerve terminals, and endogenous sympathetic histamine could inhibit itsel frelease from the nerve terminal through the presynaptic histamine H3 receptor. METHODS Using double-labeled immunohistochemistry to observe the co-localization of histamine and NE in the superior cer-vical ganglia (SCG) of macaca mulatto monkey; Different-speed centrifugation to obtain the cardiac sympathetic nerve terminal model (the cardiac synaptosomes), spectrofluorometer and ELISA techniques to detect the release of histamine from the cardiacsynaptosomes. RESULTS ( 1 ) The coexistence of histamine and norepinephrine immunoreactivities was identified in the same neuron within SCG of macaca mulatto monkey. (2) Depolarization of macaca mulatto monkey cardiac synaptosomes with 50 mmol/L potassium caused the release of endogenous histamine,