抗人精子膜蛋白抗体1对小鼠精子顶体反应和精子-透明带结合的抑制作用

• 作者: C.M.Liu C.Xu G.Y.Cheng J.L.Shi M.Wang Y.F.Wang Y.Q.Hu
• 来源期刊: 亚洲男性学杂志（英文版）
• 年，卷（期）: 2007,（1）
• 所属期刊栏目: 论著
• 页码范围: 23-30
• 页数: 8页
• 语种: 英文

摘要

Aim:To investigate the possible functions of human sperm membrane protein (hSMP-1) in the process of fertilization.Methods: A 576-bp cDNA fragment of HSD-1 gene coding for the extracellular domain of hSMP-1 was cloned and expressed. The localization of this protein on human and mouse sperm was determined by indirect immunofluorescent staining by using anti-recombinant hSMP-1 (anti-rhSMP-1) antibodies. Sperm acrosome reaction and spermzona pellucida (ZP) binding assay were carried out in 10-week-old BALB/c mice. Results: Recombinant hSMP-1 was successfully cloned and expressed. The expression of the native protein was limited on the acrosome of human and mouse sperm. Treatment of anti-rhSMP-1 antibodies significantly decreased the average number of sperms bound to each egg. Meanwhile, the percentage of acrosome reaction was decreased in comparison to pre-immune control after treatment with anti-rhSMP-1 (P ＜ 0.05). Conclusion: The results suggest that anti-rhSMP-1 antibody inhibited mouse acrosome reaction and sperm-ZP binding.