Detection of food source by PCR analysis of the gut contents of Aldrichina grahami (Aldrich) (Diptera: Calliphoridae) during post-feeding period

• 来源期刊: 中国昆虫科学(英文版)
• 关键词: Cytochrome b gene detectable time forensic entomology gut post-feeding
• 年，卷（期）: 2007,（1）
• 页码范围: 47-52
• 页数: 6页
• 语种: 英文

摘要

The last meal of sarcophagous maggots may be useful in identifying the specieson whose flesh they have fed (the"host"species). The DNA profile of the host species mayindeed be detectable in the"last meal". In this paper, mitochondrial DNA analysis of gutcontents was used to identify the prior host of post-feeding larvae of Aldrichina grahami(Aldrich) (Diptera: Calliphoridae). A modified logistic equation was fitted to estimate theprobability of identifying the host under five different constant temperatures (16, 20, 24, 28and 32℃). Our results shows that the detected time ranged from a maximum of 24 h at 32℃to 42 h at 16℃ and a minimum of 12 h at 32℃ to 30 h at 16℃. Furthermore, the host detectiontime was also calculated to give the maximal time after larval hatching from the egg. Theseresults indicate that, in criminal cases where the maggots stray from the corpse, the last mealof the larvae should not be overlooked as potentially critical evidence.