Expression of two types of acetylcholinesterase gene from the silkworm, Bombyx mori, in insect cells

• 来源期刊: 中国昆虫科学（英文版）
• 关键词: acetylcholinesterase Bmace1 Bmace2 characterization expression silkworm
• 年，卷（期）: 2007,（6）
• 页码范围: 443-450
• 页数: 8页
• 语种: 英文

摘要

Complementary DNAs encoding two types of acetylcholinesterase(AChE)were isolated from the silkworm, Bombyx mori. The type 1 (Bmace1) and type 2 (Bmace2) ORFs are 2052 and 1917 bp in length, respectively. Both the complete ORFs of the Bmaces and Cterminal truncated forms were recombined into the Bacmid baculovirus vector under the control of the polyhedrin promoter and expressed in Trichoplusia ni (Tn-5B 1-4) cells. The resulting products exhibited AChE activity and glycosylation of the expressed proteins. An inhibition assay indicated that the ace2-type enzyme was more sensitive than the acel-type enzyme to inhibition by eserine and paraoxon.