Influences of Triton X-100 on Hemoglobin Behaviors in Hemoglobin/Acyclovir/Triton X-100/H2O System

• 来源期刊: 中国化学(英文版)
• 年，卷（期）: 2007,（4）
• 页码范围: 490-497
• 页数: 8页
• 语种: 英文

摘要

The influences of Triton X-100 on hemoglobin(Hb)behaviors were studied by the methods of UV-Vis spectrum,fluorescence spectrum,HPLC,conductivity,zeta potential and negative-staining transmission electron microscope in Hb/acyclovir/Triton X-100/H2O system.With the increase of Triton X-100 concentration in the system,the percentage of the free acyclovir increased from 58%-63% to 90%-94%.The static quenching constant and the association number of acyclovir to Hb decreased.The fluorescence spectrum,conductivity,zeta potential,fluorescence polarization and negative-staining morphology of Hb tended to recover to those of the original state of Hb in the same concentration of Hb.The interaction between Triton X-100 and Hb is stronger than that between acyclovir and Hb.Most Triton-X-100 was associated with Hb at low Triton X-100 concentration.But the interaction of Triton X-100 with Hb was apparently dominant in high Triton X-100 concentration.The Hb structure was unfolded and finally denatured.