Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules

• 来源期刊: 中国物理快报（英文版）
• 年，卷（期）: 2007,（3）
• 页码范围: 644-647
• 页数: 4页
• 语种: 英文

摘要

Effects of substrate hydrophobicity/hydrophilicity on height measurement of individual ds-DNA molecules are investigated with tapping mode atomic force microscopy (TMAFM) and vibrating mode scanning polarization force microscopy (VSPFM). The measured heights of ds-DNA on hydrophobic highly oriented pyrolytic graphite (HOPG) are remarkably less than those on hydrophilic bare mica and Ni2+ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.