Preparation of a Novel COOH Ion Implantation Sensor and Its Application

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2008,（1）
• 页码范围: 130-133
• 页数: 4页
• 语种: 英文

摘要

A novel ion implantation sensor (DNA/COOH/ITO) based on DNA immobilization in COOH/ITO probe was manufactured for the first time. The surface morphologies of the electrodes were characterized by X-ray photoelectron spectroscopy (XPS), field-emission-scanning electron microscopy (FSEM) and electrochemical methods. In a 0.5 mol/L PBS solution, a sensitive oxidation peak of DNA on the COOH/ITO electrode was obtained by voltammetry. The electrochemical behavior of DNA was studied. And the oxidative peak potential of DNA was +0.400 V (vs.Ag/AgCl). Its peak current was proportional to the concentration of DNA over the range of 1.0×10-8-1.0 ×10-6 mol/L with a detection limit of 5.0×10-9 mol/L (about 0.5 ng/mL). This sensor was applied to the direct detection of DNA samples.