Optimization of the Separation of Quinolines in Micellar Liquid Chromatography by Experimental Design and Regression Models

• 来源期刊: 中国化学(英文版)
• 年，卷（期）: 2008,（12）
• 页码范围: 2197-2202
• 页数: 6页
• 语种: 英文

摘要

The chemometrics approach was applied to the optimization of separation of quinolines in micellar liquid tigated by means of multivariate analysis. The factors considered were the concentration of sodium dodecyl sulfate (SDS), the organic modifier concentration and the length of its alkyl chain, and pH of the mobile phase. The ex-periments were performed according to a face centered cube response surface experimental design. In order to op-timize the separation a Pareto-optimality method was employed. The models were verified, because a good agree-ment was observed between the predicted and experimental values of the chromatographic response function in the optimal condition. The obtained regression models were characterized by both descriptive and predictive ability (R2≥0.97 and R2cv≥0.92) and allowed the chromatographic separation of the quinolines with a good resolution and a total analysis time of 50 min.