Resonance Rayleigh Scattering Spectrum of Palladium(Ⅱ)-Clindamycin-Halofluorescein Systems and Their Analytical Applications

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2008,（10）
• 页码范围: 1879-1886
• 页数: 8页
• 语种: 英文

摘要

In pH 5.0-5.4 HOAc-NaOAc buffer solution, clindamycin (Clin) could react with Pd(II) to form a 1 : 1 cati-onic chelate, which could further react with halofluorescein dyes such as diiodofluorescein (DIP), erythrosine (Ery) and eosin Y (EY) to form 1:1 ion-association complexes. As a result, not only the absorption and fluorescence spectra were changed, but also the resonance Rayleigh scattering (RRS) intensities enhanced greatly and new RRS spectra appeared. The three reaction products had characteristic RRS spectra and their maximum RRS wavelengths were located at 285 (DIP system), 287 (Ery system) and 321 (EY system) nm, respectively. The scattering intensities were proportional to the concentration of Clin in certain range which could be applied to determine Clin. The linear ranges and detection limits of Clin were 0.025-2.1μg·mL-1 and 7.8 ng·mL-1 for the DIP system, 0.053-2.4μg·mL-1 and 16.0 ng·mL-1 for the Ery system, 0.038-2.4μg·mL-1 and 11.0 ng·mL-1 for the EY system, respectively. In this work, the optimum reaction conditions and the foreign substances were investigated. A simple, sensitive and fast method was developed for the determination of Clin either in the pharmaceutical form or in the human body fluid. Moreover, the composition, structure, reaction mechanism of the ternary complexes and their effects on the absorption, fluorescence and RRS spectra as well as the reasons of RRS enhancement were discussed.