Chiral Separation of Novel a-Aminophosphonates Containing a Benzothiazole Moiety by Liquid Chromatography Using an Amylose Stationary Phase

• 来源期刊: 中国化学(英文版)
• 年，卷（期）: 2008,（9）
• 页码范围: 1659-1665
• 页数: 7页
• 语种: 英文

摘要

The present report describes a chiral HPLC method for the enantiomeric separation of a-aminophosphonate derivatives using two new coated and immobilized amylose-based chiral stationary phases (CSP,Chiralpak IA and Chiralpak AD-H).The chromatographic parameters such as retention factor (k),separation factor (a),and resolution (Rs) of the solutes were investigated on these two CSPs.Reasonably good baseline separation for these compounds was achieved using Chiralpak IA column.The influences of temperature,content of ethanol modifier and the structure of analyte were also studied.THF,EtOAc and CH2Cl2 were used as ehients on analytical and semi-preparative columns.Highly enriched enantiomers with purities of up to 96.4%--100% (ee) and yields of 90.2%--95.5% were obtained,respectively.The proposed methods were found to be suitable and accurate for rapid separation and semi-preparation of enantiomeric a-aminophosphonate derivatives available.