A new chromatographic-method for fast separation of active proteins

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2008,（7）
• 页码范围: 1113-1116
• 页数: 4页
• 语种: 英文
• DOI: 10.1007/s11434-008-0179-7

摘要

A new method with only a single chromatographic column to accomplish the separation of intact pro-tein molecules by the combination of ion exchange chromatography and hydrophobic interaction chromatography is firstly presented. By selecting twice a suitable stationary phase, mobile phase, buffer exchange condition, and sample injection, some proteins usually required to separate with two-dimensional chromatography can now be accomplished only using the single column in one hour. In addition, the separated proteins can maintain their original three- or four-dimensional molecular structure. It would be expected that this method can also provide a new thought for sample pre-frac-tionation in proteomic investigation, especially, for the limitation of sample source.