Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology

• 作者: Ling Wang Yun Liu
• 来源期刊: 自然科学期刊(英文)
• 学科: 化学
• 关键词: Carotenoids OPTIMIZATION SOLVENT EXTRACTION Response Surface METHODOLOGY RAPESEED
• 年，卷（期）: zrkxqkyw_2009,（1）
• 页码范围: 23-29
• 页数: 7页
• 分类号: O6

摘要

The optimum total carotenoids (TC) extraction from rapeseed with solvent extraction method by UV-visible spectrophotometer determination was investigated by using response surface methodology (RSM). Extraction duration, re-peated extraction cycles, solvent-solid ratio and extraction temperature were assumed to be the most important factors affecting solvent extrac-tion for the determination of TC. Optimum sol-vent extraction conditions for maximizing the determination of TC were: extraction duration 7.3h, repeated extraction three times, ratio of solvent-solid (v/w, mL/mg) 29:1, extraction temperature 42°C. Under the optimal conditions, the yield of TC was up to 4.79 mg /100g. The model had a satisfactory coefficient of R2 (= 0.912) and verified experimentally. The results showed that the conditions were mild and use-ful for maximizing a quantitative spectropho-tometer determination of TC in rapeseed.