Cloning and characterization of a bursicon-regulated gene Su(H) in the house fly Musca domestica

• 来源期刊: 中国昆虫科学（英文版）
• 关键词: cuticle sclerotization gene regulation Musca domestica Notch pathway,r-bursicon suppressor of hairless
• 年，卷（期）: 2009,（3）
• 页码范围: 207-217
• 页数: 11页
• 语种: 英文

摘要

Bursicon is a neuropcptidc that regulates cuticle sclerotization (hardening and tanning) in insect via a G-protein coupled receptor. However, the signal transduction pathway downstream of the G-protein coupled receptor is currently not well known. In our recent microarray analysis, we identified a panel of genes regulated by bursicon in Drosophila, One of the genes, Suppressor of Hairless, or Su(H), has drawn our attention because its product acts down-stream of the bursicon receptor. In the present study, we cloned the Drosophila homolog, mdSu(H), from the house fly Muses domestics using 3' and 5' rapid amplification of complementary DNA ends. Real-time polymcrase chain reaction analysis revealed that the level ofmdSu(H) transcript is up-regulated by～3-fold I h after recombinant bursicon injection, which correlates well with the cuticle sclcrotization process obscrveei in the recombinant bursicon-injected flies. We infer that Su(H) is an essential gene involved in the insect cuticle sclerotization process.