在老鼠肝的 MD-2 和 MD-2B 表示的意义在尖锐胆管炎期间

• 作者: Hui-Lai Miao, Zhi-Dong Qiu, Fu-Long Hao, Yong-Hui Bi, Ming-Yi Li, Ming Chen, Nian-Ping Chen, Fei Zhou
• 来源期刊: 世界肝病学杂志：英文版（电子版）
• 学科: 医学
• 关键词: Acute CHOLANGITIS TOLL-LIKE receptor 4 MYELOID DIFFERENTIATION protein-2 MYELOID DIFFERENTIATION protein-2 splicing ISOFORM
• 年，卷（期）: 2010,（6）
• 页码范围: 233-238
• 页数: 6页
• 分类号: R575.7

摘要

AIM: To investigate the expression of myeloid differentiation protein-2 (MD-2), MD-2B (a splicing isoform of MD-2 that can block Toll-like receptor 4 (TLR4)/MD-2 LPS-mediated signal transduction) and TLR4 in the liver of acute cholangitis rats. METHODS: Male Sprague-Dawley rats (SPF level) were randomly divided into four groups: (A) sham-operated group; (B) simple common bile duct ligation group; (C)acute cholangitis group; and (D) acute cholangitis anti- TLR4 intervention group (n = 25 per group). Rat liver tissue samples were used to detect TLR4, MD-2 and MD-2B mRNA expression by fluorescence quantitative PCR in parallel with pathological changes. RESULTS: In acute cholangitis, liver TLR4 and MD-2 mRNA expression levels at 6, 12, 24, 48 and 72 h were gradually up-regulated but MD-2B mRNA expression gradually down-regulated (P 【 0.05). After TLR4 antibody treatment, TLR4 and MD-2 mRNA expression were lower compared with the acute cholangitis group (P 【 0.05). However, MD-2B mRNA expression was higher than in the acute cholangitis group (P 【 0.05). MD-2 and TLR4 mRNA expressions were positively correlated (r = 0.94981, P 【 0.05) and MD-2B mRNA expression was negatively correlated with MD-2 and TLR4 mRNA (r = -0.89031, -0.88997, P【 0.05). CONCLUSION: In acute cholangitis, MD-2 plays an important role in the process of TLR4- mediated in? am- matory response to liver injury while MD-2B plays a negative regulatory role.