Hepatospheres : 三种维的房间文化类似于肝的生理的条件

• 作者: Franziska van Zijl Wolfgang Mikulits
• 来源期刊: 世界肝病学杂志：英文版（电子版）
• 学科: 医学
• 关键词: Hepatosphere HEPATOCYTE TISSUE ENGINEERING 3D CULTURE MICROENVIRONMENT
• 年，卷（期）: 2010,（1）
• 页码范围: 1-7
• 页数: 7页
• 分类号: R363

摘要

Studying physiological and pathophysiological mechanisms in the liver on a molecular basis is a challenging task.During two dimensional(2D) culture conditions hepatocytes dedifferentiate rapidly by losing metabolic functions and structural integrity.Hence,inappropriate 2D hepatocellular models hamper studies on the xenobiotic metabolism of the liver which strongly influences drug potency.Also,the lack of effective therapies against hepatocellular carcinoma shows the urgent need for robust models to investigate liver functions in a defined hepatic microenvironment.Here,we summarize and discuss three-dimensional cultures of hepatocytes,herein referred to as hepatospheres,which provide versatile tools to investigate hepatic metabolism,stemness and cancer development.