在 HCV 感染和相关 hepatocellular 癌的房间周期管理者的微分表示

• 作者: Abdel-Hakeem Saad El-Deen Ahmed M Abdel-Hadi Azza E El Bassiouny Ibrahim M Ibrahim Mona K Zoheiry Mona M Nosseir Noha A Ameen Nora E El-Bassiouni Suher Zada
• 来源期刊: 世界肝病学杂志：英文版（电子版）
• 学科: 医学
• 关键词: Chronic HEPATITIS C Liver CIRRHOSIS HEPATOCELLULAR carcinoma Cell cycle CYCLIN D1 CYCLIN E p21 P27 Rb/p105
• 年，卷（期）: sjgbxzzywbdzb_2010,（1）
• 页码范围: 32-41
• 页数: 10页
• 分类号: R735.7

摘要

AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,Egypt.Fifteen wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal controls.Laboratory investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn’t express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and p27.Cyclin D1 expressed nuclear staining that was sequentially increased from CHC to LC(P【0.01) to HCC(P【0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 immuno-reactivity.The expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied cases.Correlation analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P【0.001) ,between Cyclin D1 and Cyclin E in HCCs(P【0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P【0.001) and between p21 and Rb/p105(P【0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC plays a vital role in the de