Functional analysis of hydrogenases and their effects on cell growth and magnetosome synthesis in Magnetospirillum gryphiswaldense

• 来源期刊: 科学通报(英文版)
• 学科: 生物学
• 关键词: 诱导合成 细胞生长 氢化酶 电子显微镜观察 氢气生产 螺 吸收能力 缺失突变体
• 年，卷（期）: 2010,（13）
• 所属期刊栏目: ARTICLES
• 页码范围: 1271-1277
• 页数: 7页
• 分类号: Q943.1 TQ925
• 语种: 英文

摘要

This study addressed the effect of hydrogen metabolism on cell growth and magnetosome synthesis in Magnetospirillum gryphiswaldense strain MSR-1. Two deletion mutants were generated: L206, with single deletion of the hupL gene encoding H2-uptake [NiFe] hydrogenase; and B206, with double deletion of the hyaB gene encoding H2-producing [NiFe] hydrogenase and the hupL gene. The wild-type and mutant strains were compared in terms of hydrogen uptake capability, hydrogen yield, growth rate, and iron uptake, and o...