在前面的联系房间的有免疫力的偏差的发展的 CD4+CD25+ 规章的 T 房间的效果

• 作者: Pei-Zeng Yang Shu-Xing Ji,
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: CD4+CD25+ REGULATORY T cells FOXP3 ACAID IL-10 PD-1
• 年，卷（期）: 2011,（1）
• 页码范围: 19-25
• 页数: 7页
• 分类号: R392

摘要

AIM:To investigate whether CD4+CD25+ regulatory T(Treg) cells play a role in the development of anterior chamber-associated immune deviation(ACAID).· METHODS:The dynamic changes in the frequency of CD4+CD25+ T cells,CD4+CD25+ FoxP3+ T cells and CD4+CD25+ PD-1+ T cells from spleens of mice with ACAID were analyzed by flow cytometry.Foxp3 mRNA expression in purified CD4+CD25+ T cells was analyzed using real-time PCR.The suppressive effect of purified CD4+CD25+ T cells on the proliferation of CD4+CD25-T cells was evaluated by [3H] thymidine incorporation.A blocking experiment was performed to further address the role of CD4+CD25+ T cells in ACAID.The expression of IL-10 in purified CD4+CD25+ T cells was evaluated by ELISA.· RESULTS:Increased frequencies of CD4+CD25+ T cells,CD4+CD25+ Foxp3+ T cells and CD4+CD25+ PD-1+ T cells were observed in ACAID.The CD4+CD25+ T cells from mice with ACAID showed enhanced suppressive effect on the proliferation of CD4+CD25-T cells.Treatment of BALB/c mice with anti-CD25 antibody after injection of OVA into the anterior chamber significantly inhibited the induction of ACAID.Furthermore,purified CD4+CD25+ T cells from ACAID mice secreted IL-10.· CONCLUSION:Our results demonstrate that Treg cells are induced in the mice undergoing ACAID.These Treg cells may play a role in the development of ACAID.