microRNA-16 expression in HeLa cells is upregulated in cell-cycle and drug dependent manner

• 来源期刊: 科学通报(英文版)
• 学科: 医学
• 年，卷（期）: 2011,（1）
• 页码范围: 34-38
• 页数: 5页
• 分类号: R363
• 语种: 中文

摘要

microRNAs are single-stranded,non-coding RNAs that regulate gene expression.The microRNA-16 family has been reported to be involved in cell-cycle regulation,which could also downregulate expression of multiple pro-proliferation genes.The present results demonstrated that miR-16 expression in HeLa cells increased when the cells were arrested during S-phase under methyl methanesulfate (MMS) treatment.This further resulted in downregulation of a target protein CDC25A,whereas miR-16 expression did not increase when HeLa cells were arrested during the MMS-treated G0/G1 or G2/M phase.Furthermore,when HeLa cells were arrested during S-phase with hydroxyurea treatment,miR-16 expression did not increase.These results suggest that expression levels of microRNAs in mammalian cells are delicately regulated under variable cellular conditions.