表示上的 Bak Foong 药片的效果在有眼的神经横断的老鼠视网膜淀粉

• 作者: Tan-Tai Zhao, Xiao-Jian Guo
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: BAK Foong PILLS OPTIC NERVE damage Β-AMYLOID protein BCL-2/BAX Caspase-3 mRNA
• 年，卷（期）: 2011,（1）
• 页码范围: 58-61
• 页数: 4页
• 分类号: R285.5

摘要

AIM:To investigate the effect of Bak Foong Pills(BFP) on the expression of β-amyloid(Aβ) in rats retina with optic nerve transaction,and its roles and possible mechanisms in protecting optic nerve damage.· METHODS:Seventy-two healthy,Sprague-Dawley,adult rats were randomly assigned to three groups:negative control group(control group),optic nerve transection group(model group) and BFP treatment group(BFP group,100μg/mL) followed by establishing optic nerve transection model.The expression of Aβ was measured at 48 hours by Western-blotting.Moreover,the expressions of Bcl-2,Bax and Caspase-3 mRNA were evaluated at 48 hours by reverse transcriptase polymerase chain reaction(RT-PCR).RESULTS:There were significant differences among the control,model and BFP groups in the expression of Aβ(all P 【0.01).Aβ expression was significantly higher in the model and BFP groups than that in the control group(P 【 0.01),with a more significant reduction in the BFP group than that in the model group(P 【0.01).Moreover,there were also significant differences among the three groups in the expressions of Bcl-2/Bax(Bcl-2:anti-apoptotic;Bax:proapoptotic) and Caspase-3 mRNA(proapoptotic)(all P【0.01).Bcl-2/Bax ratio was significantly lower and Caspase-3 mRNA expression was significantly higher in the model and BFP groups than those in the control group(P 【0.01),with a significant growing of Bcl-2/Bax and reduction of Caspase-3 in the BFP group than those in the model group(P【0.01).· CONCLUSION:BFP can down-regulate Aβ expression in retina and may inhibit apoptosis and protect optic nerve by enhancing Bcl-2/Bax ratio and inhibiting Caspase-3 pathway.