人的 na 的非随机的织物分发 ? ve 脐的绳索矩阵干细胞

• 作者: Dharmendra Kumar Maurya Chiyo Doi Marla Pyle Raja Shekar Rachakatla Duane Davis Masaaki Tamura Deryl Troyer
• 来源期刊: 世界干细胞杂志：英文版（电子版）
• 学科: 生物学
• 关键词: Human UMBILICAL cord matrix stem CELLS IMMUNOHISTOCHEMISTRY India black INK loaded CELLS Radio-labeled TRANSPLANTED CELLS Tissue distribution
• 年，卷（期）: 2011,（4）
• 页码范围: 34-42
• 页数: 9页
• 分类号: Q813

摘要

AIM: To determine the tissue and temporal distribution of human umbilical cord matrix stem (hUCMS) cells in severe combined immunodeficiency (SCID) mice. METHODS: For studying the localization of hUCMS cells, tritiated thymidine-labeled hUCMS cells were injected in SCID mice and tissue distribution was quantitatively determined using a liquid scintillation counter at days 1, 3, 7 and 14. Furthermore, an immunofluorescence detection technique was employed in which anti-human mitochondrial antibody was used to identify hUCMS cells in mouse tissues. In order to visualize the distribution of transplanted hUCMS cells in H&E stained tissue sections, India Black ink 4415 was used to label the hUCMS cells. RESULTS: When tritiated thymidine-labeled hUCMS cells were injected systemically (iv) in female SCID mice, the lung was the major site of accumulation at 24 h after transplantation. With time, the cells migrated to other tissues, and on day three, the spleen, stomach, and small and large intestines were the major accumulation sites. On day seven, a relatively large amount of radioactivity was detected in the adrenal gland, uterus, spleen, lung, and digestive tract. In addition, labeled cells had crossed the blood brain barrier by day 1. CONCLUSION: These results indicate that peripherally injected hUCMS cells distribute quantitatively in a tissue-specific manner throughout the body.